id: DANDI:001075/0.240920.1434 doi: 10.48324/dandi.001075/0.240920.1434 url: https://dandiarchive.org/dandiset/001075/0.240920.1434 name: Neural signal propagation atlas of Caenorhabditis elegans about: [] access: - status: dandi:OpenAccess schemaKey: AccessRequirements license: - spdx:CC-BY-4.0 version: 0.240920.1434 '@context': https://raw.githubusercontent.com/dandi/schema/master/releases/0.6.7/context.json citation: Randi, Francesco; Sharma, Anuj; Dvali, Sophie; Leifer, Andrew M. (2024) Neural signal propagation atlas of Caenorhabditis elegans (Version 0.240920.1434) [Data set]. DANDI archive. https://doi.org/10.48324/dandi.001075/0.240920.1434 keywords: - C elegans - optogenetics - functional connectivity protocol: [] schemaKey: Dandiset identifier: DANDI:001075 repository: https://dandiarchive.org contributor: - name: Baker, Cody email: cody.c.baker.phd@gmail.com roleName: - dcite:DataCurator schemaKey: Person identifier: 0000-0002-0829-4790 affiliation: [] includeInCitation: false - name: Randi, Francesco roleName: - dcite:Author - dcite:DataCollector schemaKey: Person identifier: 0000-0002-6200-7254 includeInCitation: true - name: Sharma, Anuj roleName: - dcite:Author schemaKey: Person identifier: 0000-0001-5061-9731 includeInCitation: true - name: Dvali, Sophie roleName: - dcite:Author schemaKey: Person identifier: 0000-0001-8565-7148 includeInCitation: true - url: https://phy.princeton.edu/people/andrew-leifer name: Leifer, Andrew M. email: leifer@princeton.edu roleName: - dcite:ContactPerson - dcite:Author - dcite:Conceptualization schemaKey: Person identifier: 0000-0002-5362-5093 includeInCitation: true - url: http://www.ninds.nih.gov/ name: Institutes of Health National Institute of Neurological Disorders and Stroke roleName: - dcite:Funder schemaKey: Organization identifier: https://ror.org/01s5ya894 awardNumber: DP2-NS116768 includeInCitation: false - url: https://www.simonsfoundation.org name: Simons Foundation roleName: - dcite:Funder schemaKey: Organization identifier: https://ror.org/01cmst727 awardNumber: SCGB 543003 includeInCitation: false - url: http://www.theswartzfoundation.org/ name: Swartz Foundation roleName: - dcite:Funder schemaKey: Organization identifier: https://ror.org/04eqn7j88 includeInCitation: false - url: https://www.nsf.gov name: National Science Foundation roleName: - dcite:Funder schemaKey: Organization identifier: https://ror.org/021nxhr62 awardNumber: PHY-1734030 includeInCitation: false - url: https://orip.nih.gov name: NIH Office of Research Infrastructure Programs roleName: - dcite:Funder schemaKey: Organization identifier: https://ror.org/01jdyfj45 awardNumber: P40 OD010440 includeInCitation: false dateCreated: 2024-06-27T18:17:12.716594+00:00 description: |- The dataset used in the paper "Randi, F., Sharma, A.K., Dvali, S. et al. Neural signal propagation atlas of Caenorhabditis elegans. Nature 623, 406–414 (2023)." Establishing how neural function emerges from network properties is a fundamental problem in neuroscience. Here, to better understand the relationship between the structure and the function of a nervous system, we systematically measure signal propagation in 23,433 pairs of neurons across the head of the nematode Caenorhabditis elegans by direct optogenetic activation and simultaneous whole-brain calcium imaging. We measure the sign (excitatory or inhibitory), strength, temporal properties and causal direction of signal propagation between these neurons to create a functional atlas. We find that signal propagation differs from model predictions that are based on anatomy. Using mutants, we show that extrasynaptic signalling not visible from anatomy contributes to this difference. We identify many instances of dense-core-vesicle-dependent signalling, including on timescales of less than a second, that evoke acute calcium transients—often where no direct wired connection exists but where relevant neuropeptides and receptors are expressed. We propose that, in such cases, extrasynaptically released neuropeptides serve a similar function to that of classical neurotransmitters. Finally, our measured signal propagation atlas better predicts the neural dynamics of spontaneous activity than do models based on anatomy. We conclude that both synaptic and extrasynaptic signalling drive neural dynamics on short timescales, and that measurements of evoked signal propagation are crucial for interpreting neural function. Read the paper at: https://www.nature.com/articles/s41586-023-06683-4 publishedBy: id: urn:uuid:ca50daf4-4696-4dfc-afa3-a4039b6ee489 name: DANDI publish endDate: 2024-09-20T14:34:20.438871+00:00 schemaKey: PublishActivity startDate: 2024-09-20T14:34:20.438871+00:00 wasAssociatedWith: - id: urn:uuid:cb0e3346-c4ae-478b-b7d3-61d3c552e1c3 name: DANDI API version: 0.1.0 schemaKey: Software identifier: RRID:SCR_017571 studyTarget: [] assetsSummary: species: - name: Caenorhabditis elegans schemaKey: SpeciesType identifier: http://purl.obolibrary.org/obo/NCBITaxon_6239 approach: - name: microscopy approach; cell population imaging schemaKey: ApproachType schemaKey: AssetsSummary dataStandard: - name: Neurodata Without Borders (NWB) schemaKey: StandardsType identifier: RRID:SCR_015242 numberOfBytes: 4073427051047 numberOfFiles: 223 numberOfSubjects: 113 variableMeasured: - ProcessingModule - ImagingPlane - OpticalChannel - PlaneSegmentation measurementTechnique: - name: surgical technique schemaKey: MeasurementTechniqueType - name: analytical technique schemaKey: MeasurementTechniqueType datePublished: 2024-09-20T14:34:20.438871+00:00 schemaVersion: 0.6.7 ethicsApproval: [] wasGeneratedBy: [] relatedResource: - url: https://github.com/catalystneuro/leifer_lab_to_nwb name: Leifer Lab to NWB relation: dcite:IsCompiledBy schemaKey: Resource repository: leifer_lab_to_nwb resourceType: dcite:Software - url: https://doi.org/10.1038/s41586-023-06683-4 name: Neural signal propagation atlas of Caenorhabditis elegans relation: dcite:IsDescribedBy schemaKey: Resource identifier: 10.1038/s41586-023-06683-4 resourceType: dcite:JournalArticle manifestLocation: - https://dandiarchive.s3.amazonaws.com/dandisets/001075/0.240920.1434/assets.yaml